en Occupational Health Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:10pt"><span style="line-height:150%"><span style="color:black"><b><span style="font-size:11.0pt"><span style="line-height:150%">Background:</span></span></b> <span style="font-size:11.0pt"><span style="line-height:150%">Occupational noise exposure represents the second most prevalent occupational hazard and is known to induce both auditory and extra-auditory health consequences. This study investigated the potential protective effects of pomegranate juice (PJ), as a natural antioxidant, on sex hormone levels and testicular histopathological alterations in noise-exposed rats.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="color:black"><b><span style="font-size:11.0pt"><span style="line-height:150%">Materials and Methods:</span></span></b><span style="font-size:11.0pt"><span style="line-height:150%"> In this experimental study, 64 adult male Wistar rats were randomly divided into eight groups (n = 8). After defining the control and noise groups, the remaining six groups received fresh PJ at doses of 0.5, 1, and 1.5 mL under two conditions, with and without noise exposure (100 dB). Sex hormone levels (testosterone, LH, and FSH) and testicular histopathology were assessed 50 days after treatment.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="color:black"><b><span style="font-size:11.0pt"><span style="line-height:150%">Results:</span></span></b><span style="font-size:11.0pt"><span style="line-height:150%"> Noise exposure significantly reduced the levels of all three hormones (testosterone, LH, and FSH) compared with the control group (p < 0.05). Administration of PJ at all three doses significantly increased testosterone and FSH levels in the noise-exposed groups (p < 0.05), whereas only administration of 1 and 1.5 mL of PJ significantly increased LH levels (p < 0.05). Histopathological analysis revealed that pomegranate juice (PJ) supplementation attenuated noise-induced testicular damage in the exposed groups.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="color:black"><b><span style="font-size:11.0pt"><span style="line-height:150%">Conclusions:</span></span></b><span style="font-size:11.0pt"><span style="line-height:150%"> The results indicate that noise exposure was associated with hormonal disruption and testicular damage, while PJ exerted protective effects on both parameters. Future studies should investigate the effects of pomegranate juice at multiple doses and exposure durations.</span></span></span></span></span></span></div> Noise,Pomegranate,Testicular Tissue,Sex Hormones, 109 116 http://johe.rums.ac.ir/browse.php?a_code=A-10-116-3&slc_lang=en&sid=1 Farshad Nadri Nadrifarshad64@gmail.com 22400319475328460014124 22400319475328460014124 Yes Associate Prof., Dept. of Occupational Health Engineering, Faculty of Public Health, Kermanshah University of Medical Sciences, Kermanshah, Iran. Kermanshah University of Medical Sciences Ali Tajpoor alitajpoor92@gmail.com 22400319475328460014125 22400319475328460014125 No M.Sc. in Occupational Health Engineering, Dept. of Occupational Health Engineering, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Tarbiat Modares University Hamideh Pirami hamide_pirami@yahoo.com 22400319475328460014126 22400319475328460014126 No M.Sc. in Occupational Health Engineering, Dept. of Occupational Health Engineering, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Tarbiat Modares University Hamed Nadri** Nadri_h@yahoo.com 22400319475328460014127 22400319475328460014127 No Assistant Prof., Dept. of Occupational Health Engineering, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran. Shoushtar Faculty of Medical Sciences Elaheh Tardideh elahetardideh76@gmail.com 22400319475328460014128 22400319475328460014128 No M.Sc. in Occupational Health Engineering, Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran. Kermanshah University of Medical Sciences