Volume 6, Issue 1 (Winter 2017)                   JOHE 2017, 6(1): 1-8 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Haghighat M, Khavanin A, Allameh A. Oxidative stress indices in rats’ lung tissues following simultaneous exposure to noise and styrene. JOHE. 2017; 6 (1) :1-8
URL: http://johe.rums.ac.ir/article-1-243-en.html
1- MSc Student of Occupational Health Engineering Dept. of Occupational Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
2- Associate Prof., Dept. of Occupational Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , Khavanin@modares.ac.ir
3- Professor., Dept. of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract:   (3313 Views)

Background: Simultaneous exposure to noise and organic solvents such as styrene is an indispensable part of today’s industries. Numerous studies were done related to ototoxic effects of co-exposure to noise and styrene while some evidences showed the adverse effects of such exposure on other organs. In this study, we aimed to assess the subacute effects of combined exposure to noise and styrene on rats’ lung tissue.

Materials and Methods: Twenty-four Wistar male rats were divided in four groups including: A) control, B) rats exposed to 100 dB octave band of noise, C) rats exposed to 750 ppm styrene alone, and D) rats exposed to combination of 100 dB noise and 750 ppm styrene. Following the last day of exposure, the rats were euthanized and their lung tissues were excised, homogenized and assayed for biological analysis of malondialdehyde (MDA), glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD). The results were analyzed using SPSS software.

Results: MDA increased significantly (P < 0.001) at the end of experiment in the group exposed to styrene and noise-styrene. GSH concentrations decreased significantly in styrene and noise-styrene group (P < 0.001). SOD (P < 0.001) and CAT (P < 0.05) activities were determined to be significantly lower for the styrene and noise-styrene groups compared to the control group.

Conclusions: In conclusion, our results indicated that exposure to combination of noise and styrene caused oxidative stress, increased lipid peroxidation, and antioxidant depletion. These results appear to support the fact that co-exposure to noise and styrene might cause oxidative stress-induced damage to the lung tissue. Since simultaneous exposure to noise and styrene has an additive effect in this regard, further studies are necessary to be carried out on the effects of noise and organic solvents co-exposure.

Full-Text [PDF 631 kb]   (683 Downloads) |   |   Full-Text (HTML)  (338 Views)  
Type of Study: original article | Subject: Occupational Health
Received: 2017/04/10 | Accepted: 2017/05/13 | ePublished: 2017/07/1

1. International Agency for Research on Cancer. IARC monographs on the evaluation of carcinogenic risk of chemicals to man. Wallingford, Oxfordshire, England: Centre for Agriculture and Bioscience International (CABI); 1972 Vol 1; 184p. Record Number: 19732703314. Available from: https://www.cabdirect.org/cabdirect/abstract/19732703314
2. Cohen JT, Carlson G, Charnley G, Coggon D, Delzell E, Graham JD, et al. A comprehensive evaluation of the potential health risks associated with occupational and environmental exposure to styrene. J Toxicol Environ Health B Crit Rev 2002; 5(1-2):1-263. [Article]
3. McCague AB, Cox‐Ganser JM, Harney JM, Alwis KU, Blount BC, Cummings KJ, et al. Styrene‐associated health outcomes at a windblade manufacturing plant. Am J Ind Med 2015; 58(11):1150-9. [Article]
4. Nett RJ, Cox‐Ganser JM, Hubbs AF, Ruder AM, Cummings KJ, Huang YT, et al. Non‐malignant respiratory disease among workers in industries using styrene - A review of the evidence. Am J Ind Med 2017; 60(2):163-80. [DOI] [PubMed]
5. Cruzan G, Carlson GP, Turner M, Mellert W. Ring-oxidized metabolites of styrene contribute to styrene-induced Clara-cell toxicity in mice. J Toxicol Environ Health A 2005; 68(3):229-37. [PubMed]
6. Coccini T, Fenoglio C, Nano R, Polver PDP, Moscato G, Manzo L. Styrene-induced alterations in the respiratory tract of rats treated by inhalation or intraperitoneally. J Toxicol Environ Health 1997; 52(1):63-77. [Article]
7. Mattia CJ, Ali SF, Bondy SC. Toluene-induced oxidative stress in several brain regions and other organs. Mol Chem Neuropathol 1993; 18(3):313-28. [Article]
8. Gadberry MG, DeNicola DB, Carlson GP. Pneumotoxicity and hepatotoxicity of styrene and styrene oxide. J Toxicol Environ Health 1996; 48(3):273-94. [Article]
9. Gutteridge JM, Halliwell B. Free radicals and antioxidants in the year 2000. A historical look to the future. Ann N Y Acad Sci 2000; 899:136-47. [PubMed]
10. Le Prell CG, Yamashita D, Minami ShB, Yamasoba T, Miller JM. Mechanisms of noise-induced hearing loss indicate multiple methods of prevention. Hear Res 2007; 226(1-2):22-43. [PubMed]
11. Singhal S, Yadav B, Hashmi SF, Muzammil Md. Effects of workplace noise on blood pressure and heart rate. Biomed Res 2009; 20(2):122-6. [Article]
12. Toumi ML, Merzoug S, Baudin B, Tahraoui A. Quercetin alleviates predator stress-induced anxiety-like and brain oxidative signs in pregnant rats and immune count disturbance in their offspring. Pharmacol Biochem Behav 2013; 107:1-10. [DOI] [PubMed]
13. McFadden SL, Ohlemiller KK, Ding D, Shero M, Salvi RJ. The influence of superoxide dismutase and glutathione peroxidase deficiencies on noise induced hearing loss in mice. Noise Health 2001; 3(11):49-64. [PubMed]
14. Manikandan S, Srikumar R, Jeya Parthasarathy N, Sheela Devi R. Protective effect of Acorus calamus LINN on free radical scavengers and lipid peroxidation in discrete regions of brain against noise stress exposed rat. Biol Pharm Bull 2005; 28(12):2327-30. [PubMed]
15. Miller RR, Newhook R, Poole A. Styrene production, use, and human exposure. Crit Rev Toxicol 1994; 24 Suppl:S1-10. [DOI] [PubMed]
16. Van Campen LE, Murphy WJ, Franks JR, Mathias PI, Toraason MA. Oxidative DNA damage is associated with intense noise exposure in the rat. Hear Res 2002; 164(1-2):29-38. [PubMed]
17. Lataye R, Campo P, Loquet G. Combined effects of noise and styrene exposure on hearing function in the rat. Hear Res 2000; 139(1):86-96. [PubMed]
18. Demirel R, Mollaoğlu H, Yeşilyurt H, Üçok K, Ayçiçek A, Akkaya M, et al. Noise induces oxidative stress in rat. European Journal of General Medicine 2009; 6(1):20-4. [Article]
19. Vlajkovic SM, Lin SC, Wong AC, Wackrow B, Thorne PR. Noise-induced changes in expression levels of NADPH oxidases in the cochlea. Hear Res 2013; 304:145-52. [DOI] [PubMed]
20. IACUC Guidelines: Anesthesia. Office of Animal Resources. Institutional Animal Care and Use Committee [Internet] 2016. [updated 2016 July 13]. Available from: https://animal.research.uiowa.edu/iacuc-guidelines-anesthesia
21. Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186:421-31. [PubMed]
22. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25(1):192-205. [PubMed]
23. Dhindsa RS, Plumb-Dhindsa P, Thorpe TA. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 1981; 32(1):93-101. [Article] [DOI]
24. Scandalios JG. Oxygen stress and superoxide dismutases. Plant Physiol 1993; 101(1):7-12. [PubMed]
25. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105:121-6. [PubMed]
26. Henderson D, Bielefeld EC, Harris KC, Hu BH. The role of oxidative stress in noise-induced hearing loss. Ear Hear 2006; 27(1):1-19. [DOI] [PubMed]
27. Risom L, Møller P, Loft S. Oxidative stress-induced DNA damage by particulate air pollution. Mutat Res 2005; 592(1-2):119-37. [DOI] [PubMed]
28. Bond JA. Review of the toxicology of styrene. Crit Rev Toxicol 1989; 19(3):227-49. [DOI] [PubMed]
29. Phillips DH, Farmer PB. Evidence for DNA and protein binding by styrene and styrene oxide. Crit Rev Toxicol 1994; 24 Suppl:S35-46. [DOI] [PubMed]
30. Hynes DE, DeNicola DB, Carlson GP. Metabolism of styrene by mouse and rat isolated lung cells. Toxicol Sci 1999; 51(2):195-201. [PubMed]
31. Samson J, Sheeladevi R, Ravindran R. Oxidative stress in brain and antioxidant activity of Ocimum sanctum in noise exposure. Neurotoxicology 2007; 28(3):679-85. [DOI] [PubMed]
32. Jafari MJ, Dehghani A, Khavanin A, Azari-Reza-Zade M, Dadashpourahangar A. The impact of noise and formaldehyde exposure on oxidative stress indices in blood and liver tissue of rat. International Journal of Occupational Hygiene 2014; 6(2):61-7. [Article]
33. Gamer AO, Leibold E, Deckardt K, Kittel B, Kaufmann W, Tennekes HA, et al. The effects of styrene on lung cells in female mice and rats. Food Chem Toxicol 2004; 42(10):1655-67. [PubMed]
34. Harvilchuck JA, Carlson GP. Comparison of styrene and its metabolites styrene oxide and 4-vinylphenol on cytotoxicity and glutathione depletion in Clara cells of mice and rats. Toxicology 2006; 227(1-2):165-72. [DOI] [PubMed]
35. Nathiya S, Nandhini A. Evaluation of antioxidant effect of Salacia oblonga against aluminum chloride induced visceral toxicity in albino rats. In J Basic Clin Pharmacol 2014; 3(2):315-9. [Article] [DOI]
36. Ozer EK, Goktas MT, Kilinc I, Bariskaner H, Ugurluoglu C, Iskit AB. Celecoxib administration reduced mortality, mesenteric hypoperfusion, aortic dysfunction and multiple organ injury in septic rats. Biomedicine & Pharmacotherapy 2017; 86:583-9. [Article] [DOI]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2018 All Rights Reserved | Journal of Occupational Health and Epidemiology

Designed & Developed by : Yektaweb