Volume 7, Issue 1 (Winter 2018)
J Occup Health Epidemiol 2018, 7(1): 30-36 |
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1- Department of Toxicology, School of Pharmacy, Islamic Azad University of Ahar, Ahar, Iran.
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy,Tabriz University of Medical Sciences, Tabriz, Iran.
3- Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran. , k.najafabady@sbmu.ac.ir
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy,Tabriz University of Medical Sciences, Tabriz, Iran.
3- Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran. , k.najafabady@sbmu.ac.ir
Article history
Received: 2017/10/31
Accepted: 2018/01/9
ePublished: 2018/04/16
Accepted: 2018/01/9
ePublished: 2018/04/16
Subject:
Occupational Health
Abstract: (5731 Views)
Background: Workers in different occupational positions experience significant Cu exposures, however, Cu toxicity has not been fully studied as compared to other heavy metals. In the present study, hematological and hepatic alterations have been investigated among copper mine workers.
Materials and Methods: This descriptive study was conducted in a copper mine in west of Iran, on 402 copper mine workers (study group) and 52 office employees (control group) during winter 2015. 5ml blood samples were provided from each subject and hematological and hepatic parameters including white blood cell (WBC), platelet (PLT), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCH), lymphocytes, neutrophils, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum Cu levels have been determined using their commercial kits. The results were analyzed in the SPSS software using t-test and regression method.
Results: ALT, AST, HGB, MCV, MCH and plasma Cu levels among the workers were significantly higher than the office employees (p<0.050). WBC and RBC counts among the workers were significantly lower than the control group (p= 0.049 and 0.024, respectively). Serum Cu levels of 215 of the workers were higher than its normal recommended range (120 µg/dl). For the subjects with serum Cu levels above 150 µg/dl, increases in AST and ALT were in compliance with serum Cu levels increase.
Conclusions: Significant hepatic and hematological alteration were observed among copper miners compared to control group. Employment of workers with background hematological and hepatic disorders in copper industries must be accompanied with great caution.
Materials and Methods: This descriptive study was conducted in a copper mine in west of Iran, on 402 copper mine workers (study group) and 52 office employees (control group) during winter 2015. 5ml blood samples were provided from each subject and hematological and hepatic parameters including white blood cell (WBC), platelet (PLT), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCH), lymphocytes, neutrophils, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum Cu levels have been determined using their commercial kits. The results were analyzed in the SPSS software using t-test and regression method.
Results: ALT, AST, HGB, MCV, MCH and plasma Cu levels among the workers were significantly higher than the office employees (p<0.050). WBC and RBC counts among the workers were significantly lower than the control group (p= 0.049 and 0.024, respectively). Serum Cu levels of 215 of the workers were higher than its normal recommended range (120 µg/dl). For the subjects with serum Cu levels above 150 µg/dl, increases in AST and ALT were in compliance with serum Cu levels increase.
Conclusions: Significant hepatic and hematological alteration were observed among copper miners compared to control group. Employment of workers with background hematological and hepatic disorders in copper industries must be accompanied with great caution.
References
1. Tapiero H, Townsend DM, Tew KD. Trace elements in human physiology and pathology. Copper. Biomed Pharmacother 2003; 57(9):386-98. [DOI] [PubMed]
2. Gaetke LM, Chow-Johnson HS, Chow CK. Copper: toxicological relevance and mechanisms. Arch Toxicol 2014; 88(11):1929-38. [DOI] [PMID] [PMCID]
3. Nieboer E, Thomassen Y, Romanova N, Nikonov A, Odland JØ,, Chaschin V. Multi-component assessment of worker exposures in a copper refinery. Part 2. Biological exposure indices for copper, nickel and cobalt. J Environ Monit 2007; 9(7):695-700. [DOI] [PMID]
4. Thomassen Y, Nieboer E, Romanova N, Nikanov A, Hetland S, VanSpronsen EP, et al. Multi-component assessment of worker exposures in a copper refinery Part 1. Environmental monitoring. J Environ Monit 2004; 6(12):985-91. [DOI] [PMID]
5. Pohl HR, Roney N, Abadin HG. Metal ions affecting the neurological system. Met Ions Life Sci 2010; 8:247-62. [DOI] [PubMed]
6. Klaassen CD, Watkins III JB. Casarett & Doull's essentials of toxicology. 3rd edn. New York, United States: McGraw-Hill Education; 2015. P.952-4 [Book]
7. Prasad AS. Essential and toxic element: trace elements in human health and disease. 1st ed. Cambridge, Massachusetts, United States: Academic Press; 2013. P.302-3 [Book]
8. Beshgetoor D, Hambidge M. Clinical conditions altering copper metabolism in humans. Am J Clin Nutr 1998; 67(5 Suppl):1017S-21S. [DOI] [PMID]
9. Kim SG, Kang JC. Effect of dietary copper exposure on accumulation, growth and hematological parameters of the juvenile rockfish, Sebastes schlegeli. Mar Environ Res 2004; 58(1):65-82. [DOI] [PMID]
10. Liu XJ, Luo Z, Xiong BX, Liu X, Zhao YH, Hu GF, et al. Effect of waterborne copper exposure on growth, hepatic enzymatic activities and histology in Synechogobius hasta. Ecotoxicol Environ Saf 2010; 73(6):1286-91. [DOI] [PMID]
11. Kumar V, Kalita J, Misra UK, Bora HK. A study of dose response and organ susceptibility of copper toxicity in a rat model. J Trace Elem Med Biol 2015; 29:269-74. [DOI] [PMID]
12. Sanchez W, Palluel O, Meunier L, Coquery M, Porcher JM, Ait-Aissa S. Copper-induced oxidative stress in three-spined stickleback: relationship with hepatic metal levels. Environ Toxicol Pharmacol 2005; 19(1):177-83. [DOI] [PMID]
13. Bremner I. Manifestations of copper excess. Am J Clin Nutr 1998; 67(5 Suppl):1069S-73S. [DOI] [PMID]
14. Aydemir B, Kiziler AR, Onaran I, Alici B, Ozkara H, Akyolcu MC. Impact of Cu and Fe concentrations on oxidative damage in male infertility. Biol Trace Elem Res 2006; 112(3):193-203. [DOI] [PubMed]
15. Letelier ME, Sánchez-Jofré S, Peredo-Silva L, Cortés-Troncoso J, Aracena-Parks P. Mechanisms underlying iron and copper ions toxicity in biological systems: Pro-oxidant activity and protein-binding effects. Chem Biol Interact 2010; 188(1):220-27. [DOI] [PMID]
16. Sakhaee E, Behzadi MJ, Shahrad E. Subclinical copper poisoning in asymptomatic people in residential area near copper smelting complex. Asian Pac J Trop Dis 2012; 2(6):475-7. [Sciencedirect] [DOI]
17. Singh D, Nath K, Trivedi SP, Sharma YK. Impact of copper on haematological profile of freshwater fish, Channa punctatus. J Environ Biol 2008; 29(2):253-7. [PMID]
18. Van Vuren JH, Van der Merwe M, du Preez HH. The effect of copper on the blood chemistry of Clarias gariepinus (Clariidae). Ecotoxicol Environ Saf 1994; 29(2):187-99. [DOI] [PubMed]
19. Bocca B, Madeddu R, Asara Y, Tolu P, Marchal JA, Forte G. Assessment of reference ranges for blood Cu, Mn, Se and Zn in a selected Italian population. J Trace Elem Med Biol 2011; 25(1):19-26. [DOI] [PMID]
20. Ghayour-Mobarhan M, Shapouri-Moghaddam A, Azimi-Nezhad M, Esmaeili H, Parizadeh SM, Safarian M, et al. The relationship between established coronary risk factors and serum copper and zinc concentrations in a large Persian cohort. J Trace Elem Med Biol 2009; 23(3):167-75. [DOI] [PMID]
21. Minoia C, Sabbioni E, Apostoli P, Pietra R, Pozzoli L, Gallorini M, et al. Trace element reference values in tissues from inhabitants of the European community. I. A study of 46 elements in urine, blood and serum of Italian subjects. Sci Total Environ 1990; 95:89-105. [DOI] [PubMed]
22. Nunes JA, Batista BL, Rodrigues JL, Caldas NM, Neto JA, Barbosa F Jr. A simple method based on ICP-MS for estimation of background levels of arsenic, cadmium, copper, manganese, nickel, lead, and selenium in blood of the Brazilian population. J Toxicol Environ Health A 2010; 73(13-14):878-87. [DOI] [PMID]
23. Liu J, Yang H, Shi H, Shen C, Zhou W, Dai Q, et al. Blood copper, zinc, calcium, and magnesium levels during different duration of pregnancy in Chinese. Biol Trace Elem Res 2010; 135(1-3):31-7. [DOI] [PMID]
24. Heitland P, Köster HD. Biomonitoring of 37 trace elements in blood samples from inhabitants of northern Germany by ICP–MS. J Trace Elem Med Biol 2006; 20(4):253-62. [DOI] [PMID]
25. Rosborg I, Hyllén E, Lidbeck J, Nihlgård B, Gerhardsson L. Trace element pattern in patients with fibromyalgia. Sci Total Environ 2007; 385(1-3):20-7. [DOI] [PMID]
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