Background: Morpholine is a toxic substance used in industry and agriculture and can be absorbed into the body through ingestion, inhalation, and the skin. The present study aimed to assess the effect of morpholine and physiological serum ingestion on qualitative and quantitative characteristics of white blood cells and kidney tissue of white male mice.

Materials and Methods: In the present study, 40 adult NMRI male Albino mice were placed in 4 groups control group, physiological serum (sham) group, treatment group A [fed with 300 mg/kg of 1 ml of the prepared solution (0.009 ml morpholine + 0.091 ml of distilled water) per day for 15 days], and group B (the same volume of morpholine and physiological serum). After weighing, anesthesia, and blood sampling, all considered parameters were measured using MOTIC software. In addition, macroscopic and microscopic studies were conducted on prepared slides and obtained data were analyzed using SPSS software.

Results: In group A, reduced thickness of the outer cortex of the kidney (proximal convoluted tubule), increased thickness of the inner cortex (Malpighian body’s, distal convoluted tubule), and reduced external medulla (Henle’s loop) were significant compared to the control group and sham group. However, no significant difference was found among the groups with regard to the internal medulla (collecting pipes). Moreover, the kidney gained weight compared to the whole body. Changes in white blood cells in group A were significant compared to group B.

Conclusions: Stress morpholine causes changes in blood parameters, increased filtration, decreased reabsorption and absorption, weight loss, inflammation, hyperemia, urinary tract reconstruction and resulted polyuria. However, these impacts reduced via physiological serum.