en Occupational Health Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="line-height:150%"><span courier="" new="" style="font-family:"><span style="color:black"><b><span style="font-size:11.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Background: </span></span></span></b><span style="font-size:11.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Light can be considered as one of the factors affecting performance, visual comfort, proficiency, and energy efficiency in the workplace. This study aimed to investigate the oxidative stress-induced light intensity in male rats.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span courier="" new="" style="font-family:"><span style="color:black"><b><span style="font-size:11.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Materials and Method: </span></span></span></b><span style="font-size:11.0pt"><span style="line-height:150%"><span arial="" style="font-family:">In this experimental study, a total of 32 male rats were randomly divided into four groups of eight (control group: exposure to 150 lux, group 2, 3, and 4 exposure to 300, 5000, and 8000 lux, respectively). Blood samples were collected from each rat before, 7, and 14 days after exposure. Malondialdehyde (MDA) and Total Antioxidant Capacity (TAC) levels were measured as oxidative stress indices. Data were analyzed using SPSS software.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span courier="" new="" style="font-family:"><span style="color:black"><b><span style="font-size:11.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Results:</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:150%"><span arial="" style="font-family:"> The results showed that the intensity of 8000 lux increased the MDA level and decreased the TAC level 7 and 14 days after exposure. The TAC level decreased in the group exposed to 5000 lux light intensity during 7 and 14 days (0.31&plusmn;0.065 mmol/L and 0.36&plusmn;0.077 mmol/L, respectively). The 5000 and 8000 lux light intensities decreased the TAC and caused oxidative stress in male rats after 7 and 14 days, and only the group exposed to 300 lux light intensity can restore TAC to normal levels since the 7th day.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span courier="" new="" style="font-family:"><span style="color:black"><b><span style="font-size:11.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Conclusion:</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:150%"><span arial="" style="font-family:"> The results of this study showed that light intensity is a significant factor in the development of oxidative stress. It seems that higher light intensities along with 14 days have more effects on the oxidative stress.</span></span></span></span></span></span></span></div> Oxidative Stress,Malondialdehyde,Antioxidants,Lighting, 123 129 http://johe.rums.ac.ir/browse.php?a_code=A-10-449-2&slc_lang=en&sid=1 Parvaneh Yekzamani yekzamani71@gmail.com 1 22400319475328460010776 No M.Sc in Occupational Health and Safety, Dept. of Occupational Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Iran University of Medical Sciences Iraj Alimohamadi Irajrastin1@gmail.com 2 22400319475328460010777 No Professor, Dept. of Occupational Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Iran University of Medical Sciences Jamileh Abolghasemi Abolghasemi.j@iums.ac.ir 3 22400319475328460010778 No Associated Prof., Dept. of Biostatistic, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Iran University of Medical Sciences Batol Masruri batolmasruri@gmail.com 4 22400319475328460010779 No Ph.D Student in Occupational Health and Safety, Dept. of Occupational Health Engineering, School of medical sciences, University of Tarbiat Modares, Tehran, Iran. University of Tarbiat Modares Azadeh Ashtarinezhad azadeh_ashtarinezhad@yahoo.com 5 22400319475328460010780 Yes Associated Prof., Dept. of Occupational Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Iran University of Medical Sciences