en Epidemiology Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="line-height:200%"><span courier="" new="" style="font-family:"><span style="color:black"><b><span arial="" style="font-family:">Background: </span></b><span arial="" style="font-family:">The major effects of regular exercise on diabetes mellitus are glycemic status improvement and amelioration of cardiovascular dysfunctions. This research examines whether moderate-intensity aerobic exercise can change the gene expression of metalloproteinase-2 and angiostatin in diabetic rats&#39; heart tissue.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:200%"><span courier="" new="" style="font-family:"><span style="color:black"><b><span arial="" style="font-family:">Materials & Methods:</span></b><span arial="" style="font-family:"> In this experimental study, thirty Wistar rats were categorized into the 3 groups diabetic exercise (n = 10), sedentary diabetes (n = 10), and healthy control (n = 10). To this end, moderate-intensity aerobic exercise was performed on a treadmill for 6 weeks. Real-time PCR was used to measure metalloproteinase-2 and angiostatin gene expression. serum cortisol concentrations were measured using an ELISA kit. The one-way ANOVA and Tukey&rsquo;s post-hoc test was used to check significant differences among the groups.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:200%"><span courier="" new="" style="font-family:"><span style="color:black"><b><span arial="" style="font-family:">Results</span></b><span arial="" style="font-family:">: A significant decrease in metalloproteinase-2 gene expression (P < 0/001) as well as a significant increase in angiostatin gene expression (P < 0/001) and serum cortisol concentrations (P < 0/001) were observed in the sedentary diabetes group compared to the healthy control group.</span> <span arial="" style="font-family:">Besides, a significant increase in metalloproteinase-2 gene expression (P < 0/001) as well as a significant decrease in angiostatin gene expression (P < 0/001) and serum cortisol concentrations (P < 0/001) were observed 6 weeks after the experiment in the diabetic exercise group compared to the sedentary diabetes group.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:200%"><span courier="" new="" style="font-family:"><span style="color:black"><b><span arial="" style="font-family:">Conclusions: </span></b><span arial="" style="font-family:">Aerobic exercise improved metalloproteinase-2 and angiostatin gene expression in diabetic rats&#39; heart tissue. This finding on diabetic cardiac angiogenesis factors confirms the effect&nbsp; of aerobic exercise.</span></span></span></span></span></div> Diabetes,Aerobic Exercise,Angiostatin, 265 274 http://johe.rums.ac.ir/browse.php?a_code=A-10-530-1&slc_lang=en&sid=1 Mojdeh Khajehlandi md.khajehlandi@uma.ac.ir 22400319475328460011431 22400319475328460011431 Yes Ph.D, Dept. of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran. University of Mohaghegh Ardabili Lotfali Bolboli l_bolboli@uma.ac.ir 22400319475328460011432 22400319475328460011432 No Associate Prof., Dept. of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran University of Mohaghegh Ardabili Sina Bolboli 22400319475328460011433 22400319475328460011433 No Medical Student, Faculty of Medicine, Balikesir University, Turkey. Balikesir University,