TY - JOUR JF - JOHE JO - J Occup Health Epidemiol VL - 9 IS - 4 PY - 2020 Y1 - 2020/10/01 TI - Changes of Genetic Loci among Gastric Cancer Patients, Southeastern Iran, 2017 TT - N2 - Background: Characterization of genes and precise assessment of the number of copies are crucial for understanding the basis of emergence, progression, and identification of predictive markers of tumor malignancy. This study aimed to investigate the role of the changes in some central genes in gastric cancer. Materials and Methods: In this experimental study, 30 patients with gastric surgery were selected by random sampling from four hospitals in Kerman to investigate BAX, BCL-2, P53, and MICAL-2 genes in cancerous and healthy tissues. They were then studied using real-time RT-qPCR, PCR- SSCP, and sequencing. Results: Demographic analysis demonstrated that 66.6% of patients with gastric cancer were men. The age range of men and women was 26-93 and 33-83 years, respectively. In terms of tumor pathology, there was 93.3% adenocarcinoma, 6.6% gastrointestinal stromal tumor (GIST), 16.6% intestinal type, 10% diffuse type, and 73.3% unknown type. The two exons of the p53 gene showed a 2.04 and 3.81 fold increase in expression relative to normal adjacent tissue. Results showed the upregulation of Bcl-2 (1.54%) and MICAL2 (2.23%), while the expression of Bax was downregulated (0.87%). Bcl-2/Bax ratio was not solely correlated with the progression and clinical outcome of gastric cancer. Conclusion: The data suggest that the changes in BAX, BCL2, P53, and MICAL-2 genes play a key role in gastric cancer. SP - 194 EP - 205 AU - Salari, Saeedeh AU - Noroozi Karimabad, Mojgan AU - Torkzadeh Mahani, Masoud AU - Askari, Nahid AU - Hajizadeh, Mohammad Reza AU - Mahmoodi, Mehdi AD - Prof., Dept. of Clinical Biochemistry, Afzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran; Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. KW - BAX KW - BCL2 KW - P53 KW - MICAL-2 KW - Sequencing KW - Gene UR - http://johe.rums.ac.ir/article-1-399-en.html DO - 10.52547/johe.9.4.194 ER -