Journal of Occupational Health and Epidemiology
J Occup Health Epidemiol
Medical Sciences
http://johe.rums.ac.ir
224
admin
2251-8096
2252-0902
10.61186/johe
0
en
jalali
1392
10
1
gregorian
2014
1
1
3
1
online
1
fulltext
en
Influence of morpholine on changes in kidney tissue and white blood cells of NMRI male Albino mice
Original Article
<p dir="ltr"><strong>Background:</strong> Morpholine is a toxic substance used in industry and agriculture and can be absorbed into the body through ingestion, inhalation, and the skin. The present study aimed to assess the effect of morpholine and physiological serum ingestion on qualitative and quantitative characteristics of white blood cells and kidney tissue of white male mice.</p>
<p dir="ltr"><strong>Materials and Methods:</strong> In the present study, 40 adult NMRI male Albino mice were placed in 4 groups control group, physiological serum (sham) group, treatment group A [fed with 300 mg/kg of 1 ml of the prepared solution (0.009 ml morpholine + 0.091 ml of distilled water) per day for 15 days], and group B (the same volume of morpholine and physiological serum). After weighing, anesthesia, and blood sampling, all considered parameters were measured using MOTIC software. In addition, macroscopic and microscopic studies were conducted on prepared slides and obtained data were analyzed using SPSS software.</p>
<p dir="ltr"><strong>Results:</strong> In group A, reduced thickness of the outer cortex of the kidney (proximal convoluted tubule), increased thickness of the inner cortex (Malpighian body’s, distal convoluted tubule), and reduced external medulla (Henle’s loop) were significant compared to the control group and sham group. However, no significant difference was found among the groups with regard to the internal medulla (collecting pipes). Moreover, the kidney gained weight compared to the whole body. Changes in white blood cells in group A were significant compared to group B.</p>
<p dir="ltr"><strong>Conclusions: </strong>Stress morpholine causes changes in blood parameters, increased filtration, decreased reabsorption and absorption, weight loss, inflammation, hyperemia, urinary tract reconstruction and resulted polyuria. However, these impacts reduced via physiological serum.</p>
Morpholine, White Blood Cells, Kidney
51
61
http://johe.rums.ac.ir/browse.php?a_code=A-10-122-1&slc_lang=en&sid=1
K
Zare
al.naghizadeh@yahoo.com
2240031947532846004577
2240031947532846004577
No
Dept. of Biology Science, Branch of Science and Research of Tehran Azad University, Tehran, Iran.
Tehran Azad University
HR
Adeli
adelihamidreza@yahoo.com
2240031947532846004578
2240031947532846004578
Yes
Dept. of Biology Science, Branch of Science and Research of Tehran Azad University, Tehran, Iran.
Tehran Azad University
N
Naeimi
al.naghizadeh@yahoo.com
2240031947532846004579
2240031947532846004579
No
Dept. of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran.
University of Sistan and Baluchestan
A
Naghizadeh
al.naghizadeh@yahoo.com
2240031947532846004580
2240031947532846004580
No
Dept. of Environmental Health Engineering, School of Health, Birjand University of Medical Science, Birjand, Iran.
Birjand University of Medical Science