Volume 11, Issue 4 (Autumn 2022)                   J Occup Health Epidemiol 2022, 11(4): 265-274 | Back to browse issues page


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Khajehlandi M, Bolboli L, Bolboli S. The Role of Aerobic Exercise in the Gene Expression of Metallo Proteinase-2 and Angiostatin in Diabetic Rats' Heart Tissue. J Occup Health Epidemiol 2022; 11 (4) :265-274
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1- Ph.D, Dept. of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran. , md.khajehlandi@uma.ac.ir
2- Associate Prof., Dept. of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
3- Medical Student, Faculty of Medicine, Balikesir University, Turkey.
Article history
Received: 2022/06/16
Accepted: 2022/12/1
ePublished: 2022/12/26
Subject: Epidemiology
Abstract:   (609 Views)
Background: The major effects of regular exercise on diabetes mellitus are glycemic status improvement and amelioration of cardiovascular dysfunctions. This research examines whether moderate-intensity aerobic exercise can change the gene expression of metalloproteinase-2 and angiostatin in diabetic rats' heart tissue.
Materials & Methods: In this experimental study, thirty Wistar rats were categorized into the 3 groups diabetic exercise (n = 10), sedentary diabetes (n = 10), and healthy control (n = 10). To this end, moderate-intensity aerobic exercise was performed on a treadmill for 6 weeks. Real-time PCR was used to measure metalloproteinase-2 and angiostatin gene expression. serum cortisol concentrations were measured using an ELISA kit. The one-way ANOVA and Tukey’s post-hoc test was used to check significant differences among the groups.
Results: A significant decrease in metalloproteinase-2 gene expression (P < 0/001) as well as a significant increase in angiostatin gene expression (P < 0/001) and serum cortisol concentrations (P < 0/001) were observed in the sedentary diabetes group compared to the healthy control group. Besides, a significant increase in metalloproteinase-2 gene expression (P < 0/001) as well as a significant decrease in angiostatin gene expression (P < 0/001) and serum cortisol concentrations (P < 0/001) were observed 6 weeks after the experiment in the diabetic exercise group compared to the sedentary diabetes group.
Conclusions: Aerobic exercise improved metalloproteinase-2 and angiostatin gene expression in diabetic rats' heart tissue. This finding on diabetic cardiac angiogenesis factors confirms the effect  of aerobic exercise.
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