Journal of Occupational
Health and Epidemiology
Rafsanjan university Of medical sciences
Volume 13, Issue 2 (Spring 2024)
J Occup Health Epidemiol 2024, 13(2): 62-69 |
Back to browse issues page
Ethics code: IR.AJUMS.REC.1397.517
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Hemmatipour A, Jalakani R, Jahangirimehr A, Keikhaei B, Rokhafrooz D, Rasouli Ghahfarokhi S M. Comparing Hematological Indices of People with Iron Deficiency Anemia, Thalassemia Minor and Major, and Healthy People. J Occup Health Epidemiol 2024; 13 (2) :62-69
URL: http://johe.rums.ac.ir/article-1-826-en.html
URL: http://johe.rums.ac.ir/article-1-826-en.html
Related article in
Google Scholar
Google Scholar
Similar articles
Akram Hemmatipour1 
, Razieh Jalakani2 , Azam Jahangirimehr3 , Bijan Keikhaei4 , Darush Rokhafrooz5 , Seyedeh Moloud Rasouli Ghahfarokhi * 6
, Razieh Jalakani2 , Azam Jahangirimehr3 , Bijan Keikhaei4 , Darush Rokhafrooz5 , Seyedeh Moloud Rasouli Ghahfarokhi * 6
1- M.Sc. in Nursing, Dept. of Nursing, Abadan University of Medical Sciences, Abadan, Iran.
2- M.Sc. in Hematology, Dept. of Hematology and Blood Banking, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.
3- M.Sc. in Biostatistics, Dept. of Public Health, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.
4- Associate Prof., Thalassemia & Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
5- Associate Prof., Nursing Care Research Center in Chronic Diseases, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
6- M.Sc. in Nursing, Nursing Care Research Center in Chronic Diseases, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2- M.Sc. in Hematology, Dept. of Hematology and Blood Banking, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.
3- M.Sc. in Biostatistics, Dept. of Public Health, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.
4- Associate Prof., Thalassemia & Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
5- Associate Prof., Nursing Care Research Center in Chronic Diseases, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
6- M.Sc. in Nursing, Nursing Care Research Center in Chronic Diseases, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Article history
Received: 2024/01/1
Accepted: 2024/04/20
ePublished: 2024/06/26
Accepted: 2024/04/20
ePublished: 2024/06/26
Subject:
Epidemiology
Abstract: (656 Views)
Background: This study aimed to evaluate three hematological indices in children and adolescents with iron deficiency anemia, thalassemia minor and major and healthy people.
Materials and Methods: In this descriptive cross-sectional study, 164 patients were selected based on the inclusion and exclusion criteria, and divided into four groups (n=41): iron deficiency anemia, thalassemia minor, thalassemia major, and healthy people. The results obtained from the tests were recorded in the patients' checklist. Mann-Whitney test, Wilcoxon test, ROC curve and area under the curve were adopted for data analysis using SPSS Statistics V21.0.
Results: The mean age of patients was 13.07±3.82 years, of which 100 (62.5%) were men. There is a significant difference due to the hemoglobin concentration (Hb) index in two-by-two comparison of the groups (P<0.001), but this difference was not seen between iron deficiency and thalassemia minor groups (P=0.159). This difference was substantial (P<0.001) in terms of mean corpuscular volume (MCV) index, although it was only seen between the iron deficiency and thalassemia major groups (P=0.225). Finally, this mean difference was reported in Serum ferritin index (P<0.001), but no difference was observed between healthy group and thalassemia minor group (P=0.802).
Conclusion: The results showed that there is a difference in meanHb, MCV, and Ferritin levels between the studied groups, and the Hb and Ferritin indices are favorable for iron deficiency anemia, and the Hb and MCV indices are favorable to distinguish the thalassemia minor and major from healthy people.
Materials and Methods: In this descriptive cross-sectional study, 164 patients were selected based on the inclusion and exclusion criteria, and divided into four groups (n=41): iron deficiency anemia, thalassemia minor, thalassemia major, and healthy people. The results obtained from the tests were recorded in the patients' checklist. Mann-Whitney test, Wilcoxon test, ROC curve and area under the curve were adopted for data analysis using SPSS Statistics V21.0.
Results: The mean age of patients was 13.07±3.82 years, of which 100 (62.5%) were men. There is a significant difference due to the hemoglobin concentration (Hb) index in two-by-two comparison of the groups (P<0.001), but this difference was not seen between iron deficiency and thalassemia minor groups (P=0.159). This difference was substantial (P<0.001) in terms of mean corpuscular volume (MCV) index, although it was only seen between the iron deficiency and thalassemia major groups (P=0.225). Finally, this mean difference was reported in Serum ferritin index (P<0.001), but no difference was observed between healthy group and thalassemia minor group (P=0.802).
Conclusion: The results showed that there is a difference in meanHb, MCV, and Ferritin levels between the studied groups, and the Hb and Ferritin indices are favorable for iron deficiency anemia, and the Hb and MCV indices are favorable to distinguish the thalassemia minor and major from healthy people.
Keywords: Ferritin [MeSH], Thalassemia Minor [MeSH], Beta-Thalassemia [MeSH], Iron Deficiency [MeSH], Hemoglobin Concentration [MeSH], Mean Corpuscular Volume [MeSH]
References
1. Kundu S, Alam SS, Mia MA, Hossan T, Hider P, Khalil MI, et al. Prevalence of Anemia among Children and Adolescents of Bangladesh: A Systematic Review and Meta-Analysis. Int J Environ Res Public Health. 2023 Jan 18;20(3):1786. [DOI] [PubMed] [Google Scholar]
2. Kumar SB, Arnipalli SR, Mehta P, Carrau S, Ziouzenkova O. Iron Deficiency Anemia: Efficacy and Limitations of Nutritional and Comprehensive Mitigation Strategies. Nutrients. 2022;14(14):2976. [DOI] [PMID] [PMCID]
3. Moscheo C, Licciardello M, Samperi P, La Spina M, Di Cataldo A, Russo G. New Insights into Iron Deficiency Anemia in Children: A Practical Review. Metabolites. 2022;12(4):289. [DOI] [PMID] [PMCID]
4. Wu Y, Ye H, Liu J, Ma Q, Yuan Y, Pang Q, et al. Prevalence of anemia and sociodemographic characteristics among pregnant and non-pregnant women in southwest China: a longitudinal observational study. BMC Pregnancy Childbirth. 2020;20(1):535. [DOI] [PMID] [PMCID]
5. Allali S, Brousse V, Sacri AS, Chalumeau M, de Montalembert M. Anemia in children: prevalence, causes, diagnostic work-up, and long-term consequences. Expert Rev Hematol. 2017;10(11):1023-8. [DOI] [PMID]
6. Chaparro CM, Suchdev PS. Anemia epidemiology, pathophysiology, and etiology in low- and middle-income countries. Ann N Y Acad Sci. 2019;1450(1):15-31. [DOI] [PMID] [PMCID]
7. Akbari M, Moosazadeh M, Tabrizi R, Khatibi SR, Khodadost M, Heydari ST, et al. Estimation of iron deficiency anemia in Iranian children and adolescents: a systematic review and meta-analysis. Hematology. 2017;22(4):231-9. [DOI] [PMID]
8. Kundu S, Alam SS, Mia MA, Hossan T, Hider P, Khalil MI, et al. Prevalence of Anemia among Children and Adolescents of Bangladesh: A Systematic Review and Meta-Analysis. Int J Environ Res Public Health. 2023; 20(3):1786 [DOI] [PMID] [PMCID]
9. Cheraghi SM, Rezvani A. Prevalence of Kell Ag in Pregnant Women with Thalassemia Minor or Intermedia Beta in Medical Centers Affiliated to Shiraz University of Medical Sciences in 2019-2020. J Iran Med Counc. 2022;5(4):657-60. [DOI]
10. Irankhah SS, Hoseini-Asl S, Valizadeh M, Amani F. Prevalence of RDB, RSa, Hinc and Xmn polymorphisms and Hbhaplotypes in patients with thalassemia minor. Cellular, Molecular and Biomedical Reports. 2022;2(3):162-72. [DOI]
11. Rashwan NI, Ahmed AE, Hassan MH, Mohammed ME, Bakri AH. Hematological indices in differentiation between iron deficiency anemia and beta-thalassemia trait. Int J Pediatr. 2022;10(1):15285-95. [DOI]
12. Jameel T, Baig M, Ahmed I, Hussain MB, Alkhamaly MBD. Differentiation of beta thalassemia trait from iron deficiency anemia by hematological indices. Pak J Med Sci. 2017;33(3):665-9. [DOI] [PMID] [PMCID]
13. Ullah Z, Khattak AA, Ali SA, Hussain J, Noor B, Bano R, et al. Evaluation of five discriminating indexes to distinguish Beta-Thalassemia Trait from Iron Deficiency Anaemia. J Pak Med Assoc. 2016;66(12):1627-31. [PMID]
14. Royani S, Alijanpor S, Shirbaghaei Z, Khorasaninejad R, Roshandel GH, Ayatollahi AA, et al. The Comparison of Serum Vitamin D Level in Patients with Iron Deficiency Anemia and Minor Thalassemia. Med Lab J. 2013;7(3):24-30. [Article]
15. Maleki E, Daei MH, Frahmandi Nia Z, Ebrahimi P. The Relationship between Plasma Level of 25-Hydroxyvitamin D3 and Plasma Ferritin Level in Children with Thalassemia Major. Afzalipour J Clin Res. 2017;1(1):9-18. [DOI]
16. Jahangiri M, Rahim F, Malehi AS. Diagnostic performance of hematological discrimination indices to discriminate between βeta thalassemia trait and iron deficiency anemia and using cluster analysis: Introducing two new indices tested in Iranian population. Sci Rep. 2019;9(1):18610. [DOI] [PMID] [PMCID]
17. Beyan C, Kaptan K, Ifran A. Predicive value of discrimination indices in differential diagnosis of iron deficiency anemia and beta thalassemia trait. Eur J Hematol. 2007; 78(6):524-6. [DOI] [PMID]
18. Rathod DA, Kaur A, Patel V, Patel K, Kabrawala R, Patel V, et al. Usefulness of cell counter based parameters and formulas in detection of beta thalassemia trait in areas of high prevelance. Am J Clin Pathol. 2007;128(4):585-9. [DOI] [PMID]
19. English E, Idris I, Smith G, Dhatariya K, Kilpatrick ES, John WG. The effect of anaemia and abnormalities of erythrocyte indices on HbA1c analysis: a systematic review. Diabetologia. 2015;58(7):1409-21. [DOI] [PMID]
20. Matos JF, Dusse LM, Stubbert RV, Ferreira MR, Coura-Vital W, Fernandes AP, et al. Comparison of discriminative indices for iron deficiency anemia and β thalassemia trait in a Brazilian population. Hematology. 2013;18(3):169-74. [DOI] [PMID]
21. Kramati MR, Tayyebi Meybodi N. The Effect of Iron Deficiency Anemia (IDA) on the Hb A2 Level and Comparison of Hematologic Values between IDA and Thalassemia Minor. J Intern Med Today. 2006;11(4):24-9. [Article]
22. Yousafzai YM, Khan S, Raziq F. Beta thalassemia trait: hematological parameters. J Ayub Med Coll Abbottabad. 2010; 22(4):84-6. [PMID]
23. Xiao H, Wang Y, Ye Y, Yang C, Wu X, Wu X, et al. Differential diagnosis of thalassemia and iron deficiency anemia in pregnant women using new formulas from multidimensional analysis of red blood cells. Ann Transl Med. 2021;9(2):141. [DOI] [PMID] [PMCID]
24. Dehghani M, Karimzadeh P, Azadeh N, Rezvani A, Kashkooe A. Serum Ferritin and hematological indices in thalassemia minor and nontransfusion dependent hemoghlobinopathy. Iraqi J Hematol. 2021;10(1):17-22. [DOI]
25. Tharwat RJ, Balilah S, Habib HM, Mahmoud NH, Beek FS, Almadani FK, et al. Ferritin and Vitamin D levels and its relation to bone diseases in thalassemic adults: A hospitalbased retrospective cohort study. J Appl Hematol. 2019;10(1):15-22. [DOI]
26. GUL HF, Bekis Bozkurt H, Özbolat G, Celik S. A data analysis study: is there a relationship between 25 (OH) D deficiency and iron-deficient anaemia in the pediatric population? Turk J Biochem. 2021;46(1):89-95 [DOI]
27. 27-Abdelmotaleb GS, Behairy OG, El Azim KEA, El-Hassib DMA, Hemeda TM. Assessment of serum vitamin D levels in Egyptian children with beta-thalassemia major. Egypt Pediatr Assoc Gaz. 2021;69:1-7. [DOI]
28. Zuther M, Rübsam ML, Zimmermann M, Zarbock A, Hönemann C. Improved Diagnosis of Iron Deficiency Anemia in the Critically Ill via Fluorescence Flowcytometric Hemoglobin Biomarkers. Cells. 2022;12(1):140. [DOI] [PMID] [PMCID]
29. Kablan NM, El Hamshary MO, Nasr GM, Hamza MT, Salam TH, Hemeida AA. Hemoglobin A2 Cut off Values in Egyptian Cohort as a marker of β-Thalassemia carriers. J Biosci Appl Res. 2015;1(4):154-9. [DOI]
30. Salvi CC, Braga MC, Batista Filho M. Diagnostic accuracy of hemoglobin for iron deficiency in pregnancy: disclosing results of a cited clinical trial. Rev Panam Salud Publica. 2014; 36(2):110-6. [PMID]
31. Jaime-Pérez JC, García-Arellano G, Méndez-Ramírez N, González-Llano Ó, Gómez-Almaguer D. Evaluation of hemoglobin performance in the assessment of iron stores in feto-maternal pairs in a high-risk population: receiver operating characteristic curve analysis. Rev Bras Hematol Hemoter. 2015;37(3):178-83. [DOI] [PMID] [PMCID]
32. Rivera AKB, Latorre AAE, Nakamura K, Seino K. Using complete blood count parameters in the diagnosis of iron deficiency and iron deficiency anemia in Filipino women. J Rural Med. 2023;18(2):79-86. [DOI] [PMID] [PMCID]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution 4.0 International License. |
